I-Cryopreservation yinkqantosi yamaseli ezinjengezintunu kunye nabasetyhini, kunye nama-embrasi ngenjongo yokuyigcina ixesha elingenammiselo. I-sperm, ii-oocytes kunye nama-embrasi ziyakwazi ukunyanzeliswa (kufinyelela kuma-degrees Celsius -196) kwi-nitrogen.
Ngaphambi kokuqhwala, lonke umswakama lususwa kwiiseli, kuba liyabulala xa liqhwa. Gcina izinto ezincinci kwiindawo ezikhethiweyo - iinqanawa zeDewar. Zonke izabelo zibhalwe, ii-embriyo zigcinwe kwi-vitro.
Yintoni i-cryopreservation ye-sperm nama-oocyte?
I-Sperm ingaqhwaba ukuba i-IVF i cwangciswe, kodwa ngomhla wokutyhulwa kwalo mntu akayi kuba seklinikhi ngenxa yezizathu ezithile. Esinye isikhombiso sentsholongwane esinye esinye isizathu sokuba usebenzise i-cryopreservation ye-spermatozoa. Oku kwenza ukuba kunokwenzeka kwiinqanaba eziliqela ukuqokelela inani elifanelekileyo lomdeni kwaye uphumelele inkqubo yochumiso lwe-in vitro.
Iqanda linokuqhwaba kulabo abanesifo se-oncological. Ngaphambi kokuba i-radiation kunye ne-chemotherapy, eyona nto ibangela ukuba ilahleko yokuzala inabesifazana , unokwenza isitokisi samaqanda ukwenzela ukuba esikhathini esizayo abe nezingane.
Isilwanyana esinamanzi
Iqokelelwa ukukhupha isitshixo seentsuku. Ihlaselwa isilingo sokutshatyalaliswa kunye nokuhlaselwa. Ukuba izibonakaliso zilungile, kwaye ukuvavanya ukuba kunokwenzeka yini ukuba ibombele umdaka uphumelele, izinto ezinokuqokelelwa zinyusa kwaye zandisa ingxinano, iqhutywe kakuhle kwaye ifakwe kwisitya. Isitya sinombumba ombumba weplastiki omncinane. Le bhubhu ibhalwe phantsi ukuze kungabikho iphosakelo xa ixutywe ngezilwanyana ezinqabileyo.
Ukuchithwa kwama-oocytes
Ukuqoqwa kwamaqanda ngokuqhwaba kuyinkqubo ende kwaye inzima kakhulu kunomsizi. Ibhinqa linikezelwa ukuvuselela i-hormone yama-ovari ukwenzela ukuba ii-oocytes ezininzi zivuthiwe ngexesha elifanayo. Emva koko, kukho ukugqitywa kweeqanda, ukukhethwa kwezona zinto zinokusetyenziswa. Ziyakhathazwa kwaye i-liquid excess isswe, ize ibekwe kwisitya esikhethekileyo kunye neqriziwe nge nitrogen.
I-cryopreservation yamabri
Iimbrusi zikhutshwe ngeenjongo ezininzi. Okokuqala, ukwenzela ukuba, xa kukho umzamo ongenakuphumelela, i-IVF ingahlolwa kwakhona ngaphandle kokuvuselela ii-ovari kunye nokubetha i-oocytes.
Ukongezelela, iimbumba ziphantsi kwe-cryopreservation kwiimeko apho umfazi engayinyamezeli ukutshintshwa kweembungu (i-ovarian hyperstimulation syndrome). Ukungcola kwe-endometrium yenye esinye isizathu sokubanda ama-embrosi. Iimbusi kulolu hlobo zihlakulela iintsuku eziliqela, ke ezilungileyo zazo zikhethiweyo kwaye zilungele ukunqanda. Xa i-endometrium yowesifazane ikulungele imibusi, ukudluliselwa kweembrusi ezinqabileyo zenzeka.
I-embroyo ekhuniyo igcinwa ngokukhawuleza njengoko ufuna. Enyanisweni, inkqubo yokuqhaqhaqha nokukhusekisa ingcinezelo kwimibungu. Kodwa iindlela zanamhlanje azivumeli kuphela ukugcina inani elikhulu lee-embroza eziqhenqileyo kwiimeko ezifanelekileyo, kodwa nokuqinisekisa ukuba ziqhelekile
Iimeko zokukhupha i-cryopreservation yama-embrosi
Okokuqala, kuphela ama-embrase amahle afakwa kwiqabunga - kunye neenkcukacha eziphezulu. Okwesibini, kwenzeka kwinqanaba elithile lophuhliso lwabo: kwizigaba 2, 4, 8 kunye ne-blastocysts.
Iimbusi, ezibonakaliswa zizimbi, ungaziboleki kwiqhwa, njengoko zinepropati yokuhlambalaza - ukuphuka. Maxa wambi ama-embryo amahle atshabalaliswa-le yintengo yokuqhaqhaqhafaza kunye nokulandelwa kwayo. Kodwa kukho ama-embryos amaninzi atyhile, ngoko ezinye zazo zihlala zisebenza emva kokuphazamiseka.